For all components, the design file (STL) is given if the component is home-made. All the files can be downloaded in a single file here (STL) or here (STEP). Full technical drawings of all components can be found in the supplementary information of our manuscript. If you want more detailed technical information, please mail us! For commercial components, the associated site is provided for more detail.
id | Title | Description | Image | Price |
---|---|---|---|---|
1 | Glass plate insert | CNC milled - STL file Size: 96-wells plate holder, 127.8 mm x 85.5 mm |
€500 | |
2 | XYZ stage | An ASI XY stage (requires 4 mounting holes spaced by 75 x 125 mm) with a Optophase piezo Z-insert |
€15.000 | |
3 | Objective | Nikon TIRF 1.49NA HP SR objective |
€17.500 | |
4 | Top Cover | CNC milled - STL file The Top Cover ensures correct height of the sample with respect to the parfocal distance of the objective (60 mm). |
€500 | |
5 | miCube block | CNC milled - STL file cube, STL file door The cube is milled out of solid aluminium to minimize drift. Practically all components connect to the cube in the end. |
€1.500 | |
6 | Neutral density filter | Thorlabs - NE60A-A |
€65 | |
7a | Dichroic mirror holder | Thorlabs - DFM1/M Magnetic hot-swappable dichroic mirror is placed inside. |
€330 | |
7b | Dichroic mirror | Chroma - ZT405/488/561rpc-UF2 or ZT405/488/561/640rpc-UF2 |
€500 to €550 | |
8 | TIRF filter | Chroma - ZET405/488/561m-TRF or ZET405/488/561/640m-TRF |
€900 to €950 | |
9 | Connector for dichroic mirror and mirror | Thorlabs - C4W-CC |
€45 | |
10 | 45° elliptical mirror | The fixed mirror ensures easy adjustment of position of the emission light on the camera by adjusting the two screws. | €270 | |
11 | Excitation path cover | 3D printed - STL file Ensures darkness of the setup. |
- | |
Subtotal: | €37.110 |
id | Title | Description | Image | Price |
---|---|---|---|---|
12 | Spacer | Thorlabs - SM1L05 |
€10 | |
13 | Reflective collimator holder | 3D printed - STL file Easy adjustment of height by tightening it to the mounting plate with two M6 screws. |
- | |
14 | Right-angle mounting plate | 3D printed - STL file |
- | |
15 | Translation Stage | Thorlabs - PT1/M 25mm movement for aligning the excitation laser on the back-focal plane of the objective. |
€260 | |
16 | TIRF lens | Thorlabs - AC254-200-A-ML TIRF lens to focus excitation light on back-focal plane of objective. 200mm focal length. |
€90 | |
17 | Aperture | Thorlabs - SM1D12SZ |
€70 | |
18 | Reflective collimator | Thorlabs - RC12APC-P01 A parabolic mirror in the collimator ensures a well-collimated beam simply by attaching the fibre to the collimator. |
€880 | |
- | Laserbox | Omicron - Lighthub 6 Custom laser combiner containing 4 lasers at 405 nm (60 mW), 488 nm (200 mW), 561 nm (500 mW), and 642 nm (2x 200 mW). |
€55.000 | |
Subtotal: | €56.310 |
id | Title | Description | Image | Price |
---|---|---|---|---|
19 | Camera | Andor - Zyla 4.2 PLUS sCMOS |
€15.000 | |
20 | Camera mount | 3D printed - STL file Camera mount specifically designed for the Andor Zyla 4.2 Plus sCMOS, along with the used tube lens in this setup. |
- | |
21 | Astigmatism block | 3D printed - STL file An astigmatism holder containing an astigmatism lens can be placed on two positions. |
- | |
22 | Astigmatism lens | Astigmatism lens with 1000 or 500 cm focal distance for optional super-resolution astigmatism. | €110 | |
23 | Astigmatism lens holder | 3D printed - STL file A single lens can be slot into place with a screw. A magnet can optionally be added at the bottom for a more solid placement in the block. |
- | |
24 | Astigmatism lens holder | 3D printed - STL file A single lens can be slot into place with a screw. A magnet can optionally be added at the bottom for a more solid placement in the block. |
- | |
25 | Emission path cover | Thorlabs - SC600, cut to length Ensures darkness of the microscope. |
€30 | |
26 | Connector for tube and filter block | 3D printed - STL file |
- | |
27 | Emission filter | €325 | ||
28 | Emission filter holder | 3D printed - STL file A single lens can be slot into place with a screw. A magnet can optionally be added at the bottom for a more solid placement in the holder. |
- | |
29 | Tube lens holder | 3D printed - STL file |
- | |
30 | Tube lens | Thorlabs - ITL200 200 mm focal length |
€425 | |
Subtotal: | €15.890 |
|||
Total: | €109.310 |
Id | Title | Description | Image | Price |
---|---|---|---|---|
- | DAQ | National Instruments - PCIe-6353 X Series Multifunction DAQ (32 AI, 48 DIO, 4AO), 1.25 MS/s single-channel sampling rate (781049-01) |
€3.000 + | |
- | Connector Block (2x) | National Instruments - SCB-68A Noise Rejecting, Shielded I/O Connector block (782536-01) |
€1.000 | |
- | Cables | National Instruments - SH68F-68F-EPM Cable |
€350 |
id | Title | Description | Image | Price |
---|---|---|---|---|
- | Filter holder | 3D printed - STL file Can hold up to 10 emission filters or astigmatism lenses. Has two holes at the bottom for easy placement with M6 screws. |
- | |
- | XYZ cover with LED light | 3D printed - STL files: Main lid, LED array top plate, LED array diffuser plate, Control box, Control box cover switch, indicator faceplate A single IKEA INREDA LED spot with the connector cut off is soldered to a 1/8 inch DC jack. The DC jack is connected to a circuit that controls the voltage of the spot using a potentiometer and a transistor. The schematic is depicted below (click to enlarge):
L1, L2 and L3 resemble the IKEA LED array, L4 is an indicator light for the power switch. A 16V DC switch-mode power supply (SMPS) was used. As the INREDA spot normally runs on 12V DC, R2 is placed in series with the potentiometer to limit the maximum voltage coming from the transistor Q1. The LED array is connected via J2 to J1 on the Brightfield Control Box.
|
- | |
- | Top Cover alternative | CNC milled - STL file Alternative for the normal Top Cover (nr 4). Does only hold the Z-piezo, without the XY stage. |
€500 | |
- | Breadboard | Thorlabs - MB3060/M Aluminum breadboard, 300 x 600 mm. All components (except the laser box) are placed on this breadboard. |
€240 |
id | Printer | Material | Support | Adhesion | Layer height (µm) | Top/Bottom Thickness (mm) | Wall Thickness (mm) | Infill % |
---|---|---|---|---|---|---|---|---|
13 / 14 | UM2+ | PLA | Yes | Brim (5) | 200 | 1.0 | 1.2 | 20 |
26 | UM2+ | ABS | Yes | Brim (5) | 200 | 0.8 | 0.8 | 20 |
21 | UM2+ | PLA | Yes | Brim (5) | 200 | 1.0 | 1.6 | 20 |
23 / 24 / 28 | UM2+ | PLA | Yes | Brim (5) | 200 | 1.0 | 1.6 | 20 |
20 | UM2+ | PLA | Yes | Brim (5) | 200 | 0.8 | 0.8 | 20 |